Sampling and extraction of ancient DNA from sediments

Laura S. Epp, Heike H. Zimmermann, Kathleen R. Stoof-Leichsenring

Research output: Chapter in Book/Report/Conference proceedingChapter in bookResearchpeer-review

16 Citations (Scopus)

Abstract

Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-44
Number of pages14
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1963
ISSN (Print)1064-3745

Keywords

  • Environmental DNA (eDNA)
  • Metabarcoding
  • Metagenomics
  • Permafrost
  • Sediment cores
  • sedaDNA

Programme Area

  • Programme Area 5: Nature and Climate

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