Sampling and extraction of ancient DNA from sediments

Laura S. Epp, Heike H. Zimmermann, Kathleen R. Stoof-Leichsenring

Research output: Chapter in Book/Report/Conference proceedingChapter in bookResearchpeer-review

32 Citations (Scopus)

Abstract

Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

Original languageEnglish
Title of host publicationAncient DNA
Subtitle of host publicationMethods and protocols
EditorsBeth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L.A. Paijmans, André E.R. Soares
PublisherSpringer
Pages31-44
Number of pages14
ISBN (Electronic)978-1-4939-9176-1
DOIs
Publication statusPublished - 16 Mar 2019
Externally publishedYes

Publication series

SeriesMethods in Molecular Biology
Volume1963
ISSN1064-3745

Keywords

  • Environmental DNA (eDNA)
  • Metabarcoding
  • Metagenomics
  • Permafrost
  • Sediment cores
  • sedaDNA

Programme Area

  • Programme Area 5: Nature and Climate

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