PAH degradation capacity of soil microbial communities - Does it depend on PAH exposure?

Anders R. Johnsen, Ulrich Karlson

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89 Citations (Scopus)

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the environment. But is their microbial degradation equally wide in distribution? We estimated the PAH degradation capacity of 13 soils ranging from pristine locations (total PAHs ≈ 0.1 mg kg-1) to heavily polluted industrial sites (total PAHs ≈ 400 mg kg-1). The size of the pyrene- and phenanthrene-degrading bacterial populations was determined by most probable number (MPN) enumeration. Densities of phenanthrene degraders reflected previous PAH exposure, whereas pyrene degraders were detected only in the most polluted soils. The potentials for phenanthrene and pyrene degradation were measured as the mineralization of 14C-labeled spikes. The time to 10% mineralization of added 14C phenanthrene and 14C pyrene was inversely correlated with the PAH content of the soils. Substantial 14C phenanthrene mineralization in all soils tested, including seven unpolluted soils, demonstrated that phenanthrene is not a suitable model compound for predicting PAH degradation in soils. 14C pyrene was mineralized by all Danish soil samples tested, regardless of whether they were from contaminated sites or not, suggesting that in industrialized areas the background level of pyrene is sufficient to maintain pyrene degradation traits in the gene pool of soil microorganisms. In contrast, two pristine forest soils from northern Norway and Ghana mineralized little 14C pyrene within the 140-day test period. Mineralization of phenanthrene and pyrene by all Danish soils suggests that soil microbial communities of inhabited areas possess a sufficiently high PAH degradation capacity to question the value of bioaugmentation with specific PAH degraders for bioremediation.

Original languageEnglish
Pages (from-to)488-495
Number of pages8
JournalMicrobial Ecology
Volume50
Issue number4
DOIs
Publication statusPublished - Nov 2005

Programme Area

  • Programme Area 2: Water Resources

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