New monoclonal antibody for the sensitive detection of hydroxy-s-triazines in water by enzyme-linked immunosorbent assay

Leif Bruun, Claus Koch, Mogens Havsteen Jakobsen, Jens Aamand

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

This article presents a new and sensitive immunoassay for the triazine metabolites hydroxypropazine, hydroxyatrazine and hydroxysimazine. The assay is based on covalent immobilisation of antigen in combination with an enzyme-labelled anti-hydroxy-s-triazine monoclonal antibody. This system enabled the development of an assay with variation coefficients below 3% and a detection limit below 0.01μg/l hydroxyatrazine and hydroxypropazine. Analysis of hydroxyatrazine-spiked water of three different types yielded an average recovery of 102% at 0.1μg/l hydroxyatrazine. Relative to hydroxyatrazine, assay cross-reactivity was 148% towards hydroxypropazine and 67% towards hydroxysimazine. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalAnalytica Chimica Acta
Volume423
Issue number2
DOIs
Publication statusPublished - 1 Nov 2000

Keywords

  • Covalent immobilisation
  • Herbicide
  • Hydroxytriazine
  • Immunoassay
  • Monoclonal antibody
  • Pesticide

Programme Area

  • Programme Area 2: Water Resources

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