Mineralisation of 2,6-dichlorobenzamide (BAM) in dichlobenil-exposed soils and isolation of a BAM-mineralising Aminobacter sp.

Allan Simonsen, Maria S. Holtze, Sebastian R. Sørensen, Søren J. Sørensen, Jens Aamand

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37 Citations (Scopus)

Abstract

Mineralisation of the groundwater contaminant 2,6-dichlorobenzamide (BAM), a metabolite from the herbicide 2,6-dichlorobenzonitrile (dichlobenil), was studied in soil samples obtained from 39 locations previously exposed to dichlobenil. Rapid BAM mineralisation was detected in samples from six locations with 5.2-64.6% of the added BAM mineralised within 48-50 days. From one location rapid BAM mineralisation was observed in soil samples down to a depth of 2 m below the surface. One location with fast BAM mineralisation showed significant dichlobenil degradation activity with 25.5% of the added dichlobenil being mineralised within 50 days. By inoculating soil showing the fastest mineralisation of BAM into a mineral medium with BAM as the only carbon and nitrogen source an enrichment culture was established. Community analysis based on extracted DNA revealed a change of the bacterial community but without any clear indication of key members within the BAM-mineralising culture. Parallel cultivation resulted for the first time in the isolation of a BAM-mineralising bacterium, identified as an Aminobacter sp.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalEnvironmental Pollution
Volume144
Issue number1
DOIs
Publication statusPublished - Nov 2006

Keywords

  • 2,6-Dichlorobenzamide
  • 2,6-Dichlorobenzonitrile
  • Aminobacter sp.
  • Herbicide
  • Mineralisation

Programme Area

  • Programme Area 2: Water Resources

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