Lake sedimentary dna research on past terrestrial and aquatic biodiversity: Overview and recommendations

Eric Capo, Charline Giguet-Covex, Alexandra Rouillard, Kevin Nota, Peter D. Heintzman, Aurèle Vuillemin, Daniel Ariztegui, Fabien Arnaud, Simon Belle, Stefan Bertilsson, Christian Bigler, Richard Bindler, Antony G. Brown, Charlotte L. Clarke, Sarah E. Crump, Didier Debroas, Göran Englund, Gentile Francesco Ficetola, Rebecca E. Garner, Joanna GauthierIrene Gregory-Eaves, Liv Heinecke, Ulrike Herzschuh, Anan Ibrahim, Veljo Kisand, Kurt H. Kjær, Youri Lammers, Joanne Littlefair, Erwan Messager, Marie-Eve Monchamp, Fredrik Olajos, William Orsi, Mikkel W. Pedersen, Dilli P. Rijal, Johan Rydberg, Trisha Spanbauer, Kathleen R. Stoof-Leichsenring, Pierre Taberlet, Liisi Talas, Camille Thomas, David A. Walsh, Yucheng Wang, Eske Willerslev, Anne van Woerkom, Heike H. Zimmermann, Marco J. L. Coolen, Laura S. Epp, Isabelle Domaizon, Inger G. Alsos, Laura Parducci

Research output: Contribution to journalArticleResearchpeer-review

43 Citations (Scopus)

Abstract

The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises.
Original languageEnglish
Article number6
JournalQuaternary
Volume4
Issue number1
DOIs
Publication statusPublished - 2021
Externally publishedYes

Keywords

  • sedaDNA
  • lake sediments
  • Vegetation change through time
  • Metabarcoding
  • Metagenomics
  • Paleogenomics
  • Lake sediments
  • Biodiversity
  • Paleogenetics
  • Sedimentary ancient DNA
  • Paleoecology
  • Sedimentary DNA
  • Paleolimnology

Programme Area

  • Programme Area 5: Nature and Climate

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