Intermediate accumulation of metabolites results in a bottleneck for mineralisation of the herbicide metabolite 2,6-dichlorobenzamide (BAM) by Aminobacter spp.

Allan Simonsen, Nora Badawi, Gitte G. Anskjær, Christian N. Albers, Sebastian R. Sørensen, Jan Sørensen, Jens Aamand

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24 Citations (Scopus)

Abstract

Degradation and mineralisation of the groundwater contaminant 2,6-dichloro-benzamide (BAM) was investigated in two Aminobacter strains focussing on the induction of BAM degradation and mineralisation and occurrence of intermediate metabolites. The BAM degradation rate was independent of whether the cells were pre-grown in the absence or presence of BAM, thus indicating that the first step in the degradation pathway was constitutively expressed. In contrast, 14CO2 production was stimulated when cells were pre-grown in the presence of BAM, suggesting that one or more of the subsequent steps in the degradation pathway were inducible. Accumulation of 2,6-dichlorobenzoate (DCBA) during degradation of BAM demonstrated that the first step involved amidase activity. Mass balance calculations and thin-layer chromatography coupled with autoradiographic detection indicated that degradation of DCBA and at least one unknown metabolite may comprise a bottleneck for BAM mineralisation by Aminobacter spp. The study thus provides novel information about the BAM degradation pathway and points to the involvement of unknown intermediate metabolites in degradation of this important groundwater contaminant.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume94
Issue number1
DOIs
Publication statusPublished - Apr 2012

Keywords

  • 2,6-Dichlorobenzamide
  • 2,6-Dichlorobenzoate
  • Aminobacter spp.
  • Metabolites

Programme Area

  • Programme Area 2: Water Resources

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