Archaeperidinium saanichi sp. nov.: A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex

Kenneth Neil Mertens; Aika Yamaguchi; Hisae Kawami; Sofia Ribeiro; Brian S. Leander; Andrea Michelle Price; Vera Pospelova; Marianne Ellegaard; Kazumi Matsuoka

doi:10.1016/j.marmicro.2012.08.002

Archaeperidinium saanichi sp. nov. A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex

Kenneth Neil Mertens
, Aika Yamaguchi
, Hisae Kawami
, Sofia Ribeiro
, Brian S. Leander
, Andrea Michelle Price
, Vera Pospelova
, Marianne Ellegaard
, Kazumi Matsuoka

Research output: Contribution to journal › Article › Research › peer-review

37 Citations (Scopus)

Abstract

In this paper we describe a new species, Archaeperidinium saanichi sp. nov. within the Archaeperidinium minutum Jörgensen 1912 species complex. We examined the morphological variation of the cyst and motile stage by incubation experiments from sediment samples collected in coastal British Columbia (Canada), and compared it to closely related species. The theca of A. saanichi is differentiated from related species by overall size, the asymmetry of the intercalary plates and the right-sulcal plate (S.d.) not touching the cingulum. We provide a key to differentiate all closely related species. A. saanichi can be readily distinguished from A. minutum by a distinctively large cyst with a broad 2a type archeopyle and regularly spaced processes with relatively broad bases and aculeate process tips. Molecular phylogenetic analyses of large and small subunit (LSU and SSU) rDNA sequences demonstrated a close affinity of this species to A. minutum; however, the relatively high level of sequence conservation in dinoflagellate rDNA sequences made these particular markers inadequate for distinguishing one species from the other. Sediment-trap data suggest that A. saanichi has a preference for cooler temperatures and lowered salinities.

Original language	English
Pages (from-to)	48-62
Number of pages	15
Journal	Marine Micropaleontology
Volume	96-97
DOIs	https://doi.org/10.1016/j.marmicro.2012.08.002
Publication status	Published - Dec 2012
Externally published	Yes

Keywords

Dinoflagellate
LSU rDNA
Saanich Inlet
Single-cell PCR
Spiny brown cyst
SSU rDNA

Programme Area

Programme Area 5: Nature and Climate

Access to Document

10.1016/j.marmicro.2012.08.002

Cite this

Mertens, K. N., Yamaguchi, A., Kawami, H., Ribeiro, S., Leander, B. S., Price, A. M., Pospelova, V., Ellegaard, M., & Matsuoka, K. (2012). Archaeperidinium saanichi sp. nov. A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex. Marine Micropaleontology, 96-97, 48-62. https://doi.org/10.1016/j.marmicro.2012.08.002

@article{df47a625c91a44d1a4dc2d66e038f9df,

title = "Archaeperidinium saanichi sp. nov.: A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum J{\"o}rgensen 1912 species complex",

abstract = "In this paper we describe a new species, Archaeperidinium saanichi sp. nov. within the Archaeperidinium minutum J{\"o}rgensen 1912 species complex. We examined the morphological variation of the cyst and motile stage by incubation experiments from sediment samples collected in coastal British Columbia (Canada), and compared it to closely related species. The theca of A. saanichi is differentiated from related species by overall size, the asymmetry of the intercalary plates and the right-sulcal plate (S.d.) not touching the cingulum. We provide a key to differentiate all closely related species. A. saanichi can be readily distinguished from A. minutum by a distinctively large cyst with a broad 2a type archeopyle and regularly spaced processes with relatively broad bases and aculeate process tips. Molecular phylogenetic analyses of large and small subunit (LSU and SSU) rDNA sequences demonstrated a close affinity of this species to A. minutum; however, the relatively high level of sequence conservation in dinoflagellate rDNA sequences made these particular markers inadequate for distinguishing one species from the other. Sediment-trap data suggest that A. saanichi has a preference for cooler temperatures and lowered salinities.",

keywords = "Dinoflagellate, LSU rDNA, Saanich Inlet, Single-cell PCR, Spiny brown cyst, SSU rDNA",

author = "Mertens, \{Kenneth Neil\} and Aika Yamaguchi and Hisae Kawami and Sofia Ribeiro and Leander, \{Brian S.\} and Price, \{Andrea Michelle\} and Vera Pospelova and Marianne Ellegaard and Kazumi Matsuoka",

note = "Funding Information: Kenneth Neil Mertens is a postdoctoral fellow of FWO Belgium and this research was partly conducted at Nagasaki University and was supported by a Kakenhi grant 22‐00805 . Aika Yamaguchi was supported by a postdoctoral research salary from the Assembling the Tree of Life grant (NSF \# EF-0629624 ) and operating funds to B.S.L. from the National Science and Engineering Research Council of Canada (NSERC 283091‐09 ) and the Canadian Institute for Advanced Research, Programs in Evolutionary Biology and Integrated Microbial Biodiversity . This research was partly supported by NSERC's Discovery ( 224236 ) and Ship Time grants to Vera Pospelova. Andre Catrijsse (VLIZ, Belgium) is thanked for providing us with surface sediment samples from the North Sea which unfortunately did not contain any spiny brown cysts. One Effingham Inlet sediment trap sample was kindly provided by Dr. R. Timothy Patterson (Carleton University, Canada). The VENUS (Victoria Experimental Network Under the Sea) team is thanked for their assistance with collection of Saanich Inlet sediment trap material, and surface sediments from Site A in Patricia Bay. Surface sediment samples from the NE Pacific were provided by the Scripps Institution of Oceanography (SIO), Oregon State University (OSU), Monterey Bay Aquarium Research Institute (MBARI) and U.S. Geological Survey (USGSMP). The authors like to thank the editor, Andr{\'e} Rochon and one anonymous reviewer for suggestions that significantly improved the paper. ",

year = "2012",

month = dec,

doi = "10.1016/j.marmicro.2012.08.002",

language = "English",

volume = "96-97",

pages = "48--62",

journal = "Marine Micropaleontology",

issn = "0377-8398",

publisher = "Elsevier",

}

Mertens, KN, Yamaguchi, A, Kawami, H, Ribeiro, S, Leander, BS, Price, AM, Pospelova, V, Ellegaard, M & Matsuoka, K 2012, 'Archaeperidinium saanichi sp. nov. A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex', Marine Micropaleontology, vol. 96-97, pp. 48-62. https://doi.org/10.1016/j.marmicro.2012.08.002

Archaeperidinium saanichi sp. nov. A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex. / Mertens, Kenneth Neil; Yamaguchi, Aika; Kawami, Hisae et al.
In: Marine Micropaleontology, Vol. 96-97, 12.2012, p. 48-62.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Archaeperidinium saanichi sp. nov.

T2 - A new species based on morphological variation of cyst and theca within the Archaeperidinium minutum Jörgensen 1912 species complex

AU - Mertens, Kenneth Neil

AU - Yamaguchi, Aika

AU - Kawami, Hisae

AU - Ribeiro, Sofia

AU - Leander, Brian S.

AU - Price, Andrea Michelle

AU - Pospelova, Vera

AU - Ellegaard, Marianne

AU - Matsuoka, Kazumi

N1 - Funding Information: Kenneth Neil Mertens is a postdoctoral fellow of FWO Belgium and this research was partly conducted at Nagasaki University and was supported by a Kakenhi grant 22‐00805 . Aika Yamaguchi was supported by a postdoctoral research salary from the Assembling the Tree of Life grant (NSF # EF-0629624 ) and operating funds to B.S.L. from the National Science and Engineering Research Council of Canada (NSERC 283091‐09 ) and the Canadian Institute for Advanced Research, Programs in Evolutionary Biology and Integrated Microbial Biodiversity . This research was partly supported by NSERC's Discovery ( 224236 ) and Ship Time grants to Vera Pospelova. Andre Catrijsse (VLIZ, Belgium) is thanked for providing us with surface sediment samples from the North Sea which unfortunately did not contain any spiny brown cysts. One Effingham Inlet sediment trap sample was kindly provided by Dr. R. Timothy Patterson (Carleton University, Canada). The VENUS (Victoria Experimental Network Under the Sea) team is thanked for their assistance with collection of Saanich Inlet sediment trap material, and surface sediments from Site A in Patricia Bay. Surface sediment samples from the NE Pacific were provided by the Scripps Institution of Oceanography (SIO), Oregon State University (OSU), Monterey Bay Aquarium Research Institute (MBARI) and U.S. Geological Survey (USGSMP). The authors like to thank the editor, André Rochon and one anonymous reviewer for suggestions that significantly improved the paper.

PY - 2012/12

Y1 - 2012/12

N2 - In this paper we describe a new species, Archaeperidinium saanichi sp. nov. within the Archaeperidinium minutum Jörgensen 1912 species complex. We examined the morphological variation of the cyst and motile stage by incubation experiments from sediment samples collected in coastal British Columbia (Canada), and compared it to closely related species. The theca of A. saanichi is differentiated from related species by overall size, the asymmetry of the intercalary plates and the right-sulcal plate (S.d.) not touching the cingulum. We provide a key to differentiate all closely related species. A. saanichi can be readily distinguished from A. minutum by a distinctively large cyst with a broad 2a type archeopyle and regularly spaced processes with relatively broad bases and aculeate process tips. Molecular phylogenetic analyses of large and small subunit (LSU and SSU) rDNA sequences demonstrated a close affinity of this species to A. minutum; however, the relatively high level of sequence conservation in dinoflagellate rDNA sequences made these particular markers inadequate for distinguishing one species from the other. Sediment-trap data suggest that A. saanichi has a preference for cooler temperatures and lowered salinities.

AB - In this paper we describe a new species, Archaeperidinium saanichi sp. nov. within the Archaeperidinium minutum Jörgensen 1912 species complex. We examined the morphological variation of the cyst and motile stage by incubation experiments from sediment samples collected in coastal British Columbia (Canada), and compared it to closely related species. The theca of A. saanichi is differentiated from related species by overall size, the asymmetry of the intercalary plates and the right-sulcal plate (S.d.) not touching the cingulum. We provide a key to differentiate all closely related species. A. saanichi can be readily distinguished from A. minutum by a distinctively large cyst with a broad 2a type archeopyle and regularly spaced processes with relatively broad bases and aculeate process tips. Molecular phylogenetic analyses of large and small subunit (LSU and SSU) rDNA sequences demonstrated a close affinity of this species to A. minutum; however, the relatively high level of sequence conservation in dinoflagellate rDNA sequences made these particular markers inadequate for distinguishing one species from the other. Sediment-trap data suggest that A. saanichi has a preference for cooler temperatures and lowered salinities.

KW - Dinoflagellate

KW - LSU rDNA

KW - Saanich Inlet

KW - Single-cell PCR

KW - Spiny brown cyst

KW - SSU rDNA

UR - https://www.scopus.com/pages/publications/84865702406

U2 - 10.1016/j.marmicro.2012.08.002

DO - 10.1016/j.marmicro.2012.08.002

M3 - Article

AN - SCOPUS:84865702406

SN - 0377-8398

VL - 96-97

SP - 48

EP - 62

JO - Marine Micropaleontology

JF - Marine Micropaleontology