Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen; Jim Rasmussen; Carsten S. Jacobsen; Ole S. Jacobsen; René K. Juhler; Jens Aamand

doi:10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen
, Jim Rasmussen
, Carsten S. Jacobsen
, Ole S. Jacobsen
, René K. Juhler
, Jens Aamand

Department of Geochemistry

Research output: Contribution to journal › Article › Research › peer-review

65 Citations (Scopus)

Abstract

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- ¹⁴C]linuron completely to ¹⁴CO₂ and ¹⁴C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Original language	English
Pages (from-to)	4144-4148
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	7
DOIs	https://doi.org/10.1128/AEM.71.7.4144-4148.2005
Publication status	Published - Jul 2005

Programme Area

Programme Area 2: Water Resources

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Sorensen, S. R., Rasmussen, J., Jacobsen, C. S., Jacobsen, O. S., Juhler, R. K., & Aamand, J. (2005). Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology, 71(7), 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

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title = "Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation",

abstract = "A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.",

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Sorensen, SR, Rasmussen, J, Jacobsen, CS, Jacobsen, OS, Juhler, RK & Aamand, J 2005, 'Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation', Applied and Environmental Microbiology, vol. 71, no. 7, pp. 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. / Sorensen, Sebastian R.; Rasmussen, Jim; Jacobsen, Carsten S. et al.
In: Applied and Environmental Microbiology, Vol. 71, No. 7, 07.2005, p. 4144-4148.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

AU - Sorensen, Sebastian R.

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AU - Juhler, René K.

AU - Aamand, Jens

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AB - A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

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DO - 10.1128/AEM.71.7.4144-4148.2005

M3 - Article

SN - 0099-2240

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SP - 4144

EP - 4148

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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Sorensen SR, Rasmussen J, Jacobsen CS, Jacobsen OS, Juhler RK, Aamand J. Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology. 2005 Jul;71(7):4144-4148. doi: 10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

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