Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen; Jim Rasmussen; Carsten S. Jacobsen; Ole S. Jacobsen; René K. Juhler; Jens Aamand

doi:10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen, Jim Rasmussen, Carsten S. Jacobsen, Ole S. Jacobsen, René K. Juhler, Jens Aamand

Department of Geochemistry

Research output: Contribution to journal › Article › Research › peer-review

65 Citations (Scopus)

Abstract

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- ¹⁴C]linuron completely to ¹⁴CO₂ and ¹⁴C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Original language	English
Pages (from-to)	4144-4148
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	7
DOIs	https://doi.org/10.1128/AEM.71.7.4144-4148.2005
Publication status	Published - Jul 2005

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Programme Area 2: Water Resources

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Sorensen, S. R., Rasmussen, J., Jacobsen, C. S., Jacobsen, O. S., Juhler, R. K., & Aamand, J. (2005). Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology, 71(7), 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

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abstract = "A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.",

author = "Sorensen, {Sebastian R.} and Jim Rasmussen and Jacobsen, {Carsten S.} and Jacobsen, {Ole S.} and Juhler, {Ren{\'e} K.} and Jens Aamand",

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Sorensen, SR, Rasmussen, J, Jacobsen, CS, Jacobsen, OS, Juhler, RK & Aamand, J 2005, 'Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation', Applied and Environmental Microbiology, vol. 71, no. 7, pp. 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. / Sorensen, Sebastian R.; Rasmussen, Jim; Jacobsen, Carsten S. et al.
In: Applied and Environmental Microbiology, Vol. 71, No. 7, 07.2005, p. 4144-4148.

Research output: Contribution to journal › Article › Research › peer-review

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AB - A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

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Sorensen SR, Rasmussen J, Jacobsen CS, Jacobsen OS, Juhler RK, Aamand J. Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology. 2005 Jul;71(7):4144-4148. doi: 10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

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