Development and dynamics of Pseudomonas sp biofilms

Tim Tolker-Nielsen, Ulla C. Brinch, Paula C. Ragas, Jens Bo. Andersen, Carsten Suhr Jacobsen, Søren Molin

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)


Pseudomonas sp. strain B13 and Pseudomonas putida OUS82 were genetically tagged with the green fluorescent protein and the Discosoma sp. red fluorescent protein, and the development and dynamics occurring in flow chamber-grown two-colored monospecies or mixed-species biofilms were investigated by the use of confocal scanning laser microscopy. Separate red or green fluorescent microcolonies were formed initially, suggesting that the initial small microcolonies were formed simply by growth of substratum attached cells and not by cell aggregation. Red fluorescent microcolonies containing a few green fluorescent cells and green fluorescent microcolonies containing a few red fluorescent cells were frequently observed in both monospecies and two-species biofilms, suggesting that the bacteria moved between the microcolonies. Rapid movement of P. putida OUS82 bacteria inside microcolonies was observed before a transition from compact microcolonies to loose irregularly shaped protruding structures occurred. Experiments involving a nonflagellated P. putida OUS82 mutant suggested that the movements between and inside microcolonies were flagellum driven. The results are discussed in relation to the prevailing hypothesis that biofilm bacteria are in a physiological state different from planktonic bacteria.

Original languageEnglish
Pages (from-to)6482-6489
Number of pages8
JournalJournal of Bacteriology
Issue number22
Publication statusPublished - 2000

Programme Area

  • Programme Area 2: Water Resources


Dive into the research topics of 'Development and dynamics of Pseudomonas sp biofilms'. Together they form a unique fingerprint.

Cite this