Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

Carsten S. Jacobsen

doi:10.1007/978-1-4020-2177-0_2

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

Carsten S. Jacobsen

Department of Geochemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter in book › Research › peer-review

Abstract

Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil
microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

Original language	English
Title of host publication	Molecular microbial ecology manual (Second edition)
Editors	George A. Kowalchuk, Frans J. de Bruijn, Ian M. Head, Antoon D.L. Akkermans, Jan Dirk van Elsas
Place of Publication	Dordrecht, The Netherlands
Publisher	Kluwer Academic Publishers
Chapter	2.08
Pages	333-344
Number of pages	12
Edition	2
ISBN (Electronic)	978-1-4020-2177-0
ISBN (Print)	978-1-4020-2176-3
DOIs	https://doi.org/10.1007/978-1-4020-2177-0_2
Publication status	Published - 2004

Programme Area

Programme Area 2: Water Resources

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10.1007/978-1-4020-2177-0_2

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Jacobsen, C. S. (2004). Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. In G. A. Kowalchuk, F. J. de Bruijn, I. M. Head, A. D. L. Akkermans, & J. D. van Elsas (Eds.), Molecular microbial ecology manual (Second edition) (2 ed., pp. 333-344). Kluwer Academic Publishers. https://doi.org/10.1007/978-1-4020-2177-0_2

Jacobsen, Carsten S. / Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. Molecular microbial ecology manual (Second edition). editor / George A. Kowalchuk ; Frans J. de Bruijn ; Ian M. Head ; Antoon D.L. Akkermans ; Jan Dirk van Elsas. 2. ed. Dordrecht, The Netherlands : Kluwer Academic Publishers, 2004. pp. 333-344

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abstract = "Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.",

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Jacobsen, CS 2004, Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. in GA Kowalchuk, FJ de Bruijn, IM Head, ADL Akkermans & JD van Elsas (eds), Molecular microbial ecology manual (Second edition). 2 edn, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 333-344. https://doi.org/10.1007/978-1-4020-2177-0_2

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. / Jacobsen, Carsten S.
Molecular microbial ecology manual (Second edition). ed. / George A. Kowalchuk; Frans J. de Bruijn; Ian M. Head; Antoon D.L. Akkermans; Jan Dirk van Elsas. 2. ed. Dordrecht, The Netherlands: Kluwer Academic Publishers, 2004. p. 333-344.

Research output: Chapter in Book/Report/Conference proceeding › Chapter in book › Research › peer-review

TY - CHAP

T1 - Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

AU - Jacobsen, Carsten S.

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N2 - Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

AB - Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

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DO - 10.1007/978-1-4020-2177-0_2

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SP - 333

EP - 344

BT - Molecular microbial ecology manual (Second edition)

A2 - Kowalchuk, George A.

A2 - de Bruijn, Frans J.

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PB - Kluwer Academic Publishers

CY - Dordrecht, The Netherlands

ER -

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

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