Degradation of PAHs in soil by indigenous and inoculated bacteria

J. Aamand, G. Bruntse, M. Jepsen, C. Jørgensen, B.K. Jensen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedings

Abstract

In soil heavily polluted by coal tar, the inherent mineralization of radio-labeled phenanthrene to {sup 14}CO{sub 2} was relatively slow, and a stimulation of degradation was observed by inoculation with a mixed population of PAH-degrading bacteria. A much faster inherent mineralization of phenanthrene was observed in soil slightly polluted by coal tar, and inoculation of this soil had no effect. Several phenanthrene-degrading bacteria were isolated from different soils. Two strains were further characterized as an Arthrobacter sp. and a Pseudomonas sp. In an organic medium without phenanthrene, growth rates of 0.52 h{sup {minus}1} and 0.71 h{sup {minus}1} were measured for the Arthrobacter sp. and the Pseudomonas sp., respectively. Most isolates grown in the phenanthrene-free medium, including the Arthrobacter sp., rapidly adapted to phenanthrene degradation following transfer to a phenanthrene-containing medium. In contrast, the phenanthrene-degrading capability of other strains, including the Pseudomonas sp., was lost during growth in the phenanthrene-free medium. Growth in an organic medium without phenanthrene of strains that retain the ability to degrade phenanthrene could prove to be a useful technique for production of PAH-degrading bacteria on a larger scale for soil inoculation.
Original languageEnglish
Title of host publicationBioaugmentation for site remediation
EditorsRobert E. Hinchee, Jim Fredrickson, Bruce C. Alleman
PublisherBattelle
Pages121-127
Number of pages7
ISBN (Print)1-57477-004-7, 978-3540724476
Publication statusPublished - 1995
Event3rd International In Situ and On-Site Bioreclamation Symposium - San Diego, USA
Duration: 1 Apr 1995 → …

Publication series

SeriesBioremediation Series
Volume3

Conference

Conference3rd International In Situ and On-Site Bioreclamation Symposium
CitySan Diego, USA
Period1/04/95 → …

Programme Area

  • Programme Area 2: Water Resources

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