A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format

Anders R. Johnsen; Trine K. Hybholt; Ole S. Jacobsen; Jens Aamand

doi:10.1016/j.mimet.2009.07.018

A radiorespirometric method for measuring mineralization of [¹⁴C]-compounds in a 96-well microplate format

Anders R. Johnsen
, Trine K. Hybholt
, Ole S. Jacobsen
, Jens Aamand

Department of Geochemistry

Research output: Contribution to journal › Article › Research › peer-review

17 Citations (Scopus)

Abstract

A radiorespirometric method was developed for quantifying mineralization of [¹⁴C]-labeled substrates in deep-well microplates. Ninety-six ¹⁴CO₂-traps were placed on an adhesive microplate sealing tape which made it possible to trap ¹⁴CO₂ simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

Original language	English
Pages (from-to)	114-116
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2009.07.018
Publication status	Published - Oct 2009

Keywords

Autoradiography
Mineralization
MPN
Phenanthrene
Soil

Programme Area

Programme Area 2: Water Resources

Access to Document

10.1016/j.mimet.2009.07.018

Cite this

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title = "A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format",

abstract = "A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.",

keywords = "Autoradiography, Mineralization, MPN, Phenanthrene, Soil",

author = "Johnsen, \{Anders R.\} and Hybholt, \{Trine K.\} and Jacobsen, \{Ole S.\} and Jens Aamand",

note = "Funding Information: This work was funded by the Villum Kann Rasmussen Foundation via the Center for Environmental and Agricultural Microbiology (CREAM).",

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AU - Johnsen, Anders R.

AU - Hybholt, Trine K.

AU - Jacobsen, Ole S.

AU - Aamand, Jens

N1 - Funding Information: This work was funded by the Villum Kann Rasmussen Foundation via the Center for Environmental and Agricultural Microbiology (CREAM).

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AB - A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

KW - Autoradiography

KW - Mineralization

KW - MPN

KW - Phenanthrene

KW - Soil

UR - https://www.scopus.com/pages/publications/70349216333

U2 - 10.1016/j.mimet.2009.07.018

DO - 10.1016/j.mimet.2009.07.018

M3 - Article

C2 - 19635506

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EP - 116

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