Sampling and extraction of ancient DNA from sediments

Laura S. Epp, Heike H. Zimmermann, Kathleen R. Stoof-Leichsenring

Publikation: Bidrag til bog/rapport/konferenceproceedingsBogkapitelForskningpeer review

16 Citationer (Scopus)

Abstrakt

Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.

OriginalsprogEngelsk
TitelMethods in Molecular Biology
ForlagHumana Press Inc.
Sider31-44
Antal sider14
DOI
StatusUdgivet - 2019
Udgivet eksterntJa

Publikationsserier

NavnMethods in Molecular Biology
Vol/bind1963
ISSN (Trykt)1064-3745

Programområde

  • Programområde 5: Natur og klima

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