Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts: methodological framework and applications

Pjotr Meyvisch, Pieter Roger Gurdebeke, Henk Vrielinck, Kenneth Neil Mertens, Gerard Versteegh, Katarzyna Sliwinska, Stephen Louwye

    Publikation: Bidrag til bog/rapport/konferenceproceedingsAbstract i proceedings

    Abstrakt

    Fourier-transformed infrared (FTIR) spectroscopy is a spectrochemical technique able to retrieve macromolecular information from organic materials. When combined with a microscope (micro-FTIR), the (geo)chemical composition of single specimen dinoflagellate cysts (dinocysts) can be determined. Over the last decades a small number of dinocyst micro-FTIR studies booked often inconsistent results by overlooking important methodological aspects during analysis. This study takes into account variables like sample preparation, specimen morphology and size and spectral data processing steps and presents a standardized method based on attenuated total reflectance (ATR) micro-FTIR spectroscopy which is able to collect robust spectral datasets. These datasets are largely devoid of nonchemical artifacts inherent to other infrared spectrochemical methods which have typically been used in similar studies in the past (i.e. transmission and transflection spectroscopy). Several guidelines are proposed which facilitate the collection and qualitative interpretation of highly reproducible and repeatable spectrochemical dinocyst data. These, in turn, pave the way for a systematic exploration of dinocyst chemistry and its assessment as a chemotaxonomical tool or proxy. An ATR micro-FTIR case study on morphologically similar late Paleogene to early Neogene dinocysts Palaeocystodinium golzowense, Svalbardella clausii and Svalbardella cooksoniae is also presented, which highlights the chemotaxonomical potential of the method.
    OriginalsprogEngelsk
    TitelGeosciences made in Belgium
    UndertitelConference program & abstract book
    RedaktørerDamien Delvaux, Olivier Dewitte, Max Fernandez-Alonso, Aurelia Hubert-Ferrari, Jan Elsen
    UdgivelsesstedBruxelles
    ForlagGeologica Belgica
    Sider249
    Antal sider1
    ISBN (Trykt)978-2-9601402-3-1
    DOI
    StatusUdgivet - sep. 2021
    Begivenhed7th International Geologica Belgica Meeting: Geosciences made in Belgium - AfricaMuseum, Tervuren, Belgien
    Varighed: 15 sep. 202117 sep. 2021
    Konferencens nummer: 7th

    Publikationsserier

    NavnGeologica Belgica Conference Proceedings
    ForlagGeologica Belgica
    Vol/bind4
    ISSN (Elektronisk)2593-6670

    Konference

    Konference7th International Geologica Belgica Meeting
    Land/OmrådeBelgien
    ByTervuren
    Periode15/09/2117/09/21

    Programområde

    • Programområde 5: Natur og klima

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