Purification and structural analysis of a type III antifreeze protein from the European eelpout Zoarces viviparus

C.N. Albers, M. Bjørn-Mortensen, P.E. Hansen, H. Ramløv, Thomas Sørensen

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

5 Citationer (Scopus)

Abstrakt

It has recently been reported that the eelpout Zoarces viviparus synthesizes a family of antifreeze proteins (AFP) similar in sequence to type III AFPs. A method has been set up to separate these antifreeze proteins from blood serum of this teleost species. A total of nine proteins with antifreeze activity have been isolated, several to a purity suited for NMR experiments. One of the proteins, Zvafp13, has been subject to partial structure determination by NMR. 1D- and 2D-1H NMR analyses were carried out. In the 1D-experiments it was observed that the protein contained 28 slow-exchanging amides, suggesting a compact structure. The 2D-experiments were utilized to assign observed signals to specific amino acids. From TOCSY- and NOESY-experiments 35 out of a total of 66 amino acids were assigned. The amide exchange pattern, protein primary sequence, chemical shifts and NOE-cross-peaks between amides and α-protons in the β-sheets suggest that Zvafp13 structurally resembles the recombinant type III AFP rQAE m1.1.

OriginalsprogEngelsk
Sider (fra-til)51-60
Antal sider10
TidsskriftCryo-Letters
Vol/bind28
Udgave nummer1
StatusUdgivet - jan. 2007
Udgivet eksterntJa

Programområde

  • Programområde 5: Natur og klima

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