Resumé
This article presents a new and sensitive immunoassay for the triazine metabolites hydroxypropazine, hydroxyatrazine and hydroxysimazine. The assay is based on covalent immobilisation of antigen in combination with an enzyme-labelled anti-hydroxy-s-triazine monoclonal antibody. This system enabled the development of an assay with variation coefficients below 3% and a detection limit below 0.01μg/l hydroxyatrazine and hydroxypropazine. Analysis of hydroxyatrazine-spiked water of three different types yielded an average recovery of 102% at 0.1μg/l hydroxyatrazine. Relative to hydroxyatrazine, assay cross-reactivity was 148% towards hydroxypropazine and 67% towards hydroxysimazine. Copyright (C) 2000 Elsevier Science B.V.
Originalsprog | Engelsk |
---|---|
Sider (fra-til) | 205-213 |
Antal sider | 9 |
Tidsskrift | Analytica Chimica Acta |
Vol/bind | 423 |
Udgave nummer | 2 |
DOI | |
Status | Udgivet - 1 nov. 2000 |
Emneord
- Immunoassay
- Pesticide
Programområde
- Programområde 2: Vandressourcer