Pseudomonas frederiksbergensis sp nov., isolated from soil at a coal gasification site

Søren M. Andersen, Kaare Johnsen, Jan Sørensen, Preben Nielsen, Carsten S. Jacobsen

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Abstrakt

Phenotypic and genotypic characterization indicated that a group of 29 closely related phenanthrene-degrading bacteria from a coal gasification site in Frederiksberg, Copenhagen, Denmark, belonged to the genus Pseudomonas. The strains were isolated at two sampling occasions 2 years apart. The isolates were phenotypically different from any known species of the genus Pseudomonas and were therefore subject to further identification. Colonies were smooth and pale yellowish and did not produce pigments fluorescent in UV light when grown on King's B agar. Cells were rod shaped, approximately 0·5-0·8 × 1·5-3·0 μm, and grew at 4 and 30 °C, but not 37 °C. The bacteria were oxidase- and catalase positive, accumulated poly-β-hydroxybutyrate and denitrified, but did not utilize D-xylose. The mean G+C content was 59·6 mol%. Phenotypic data and 16S rDNA sequence data information for Pseudomonas amygdali and Pseudomonas corrugata, and 16S rDNA sequence data for Pseudomonas chlororaphis and Pseudomonas syringae showed close relationships to these strains. However, DNA-DNA hybridization data showed that the isolates belong to a new species, for which the name Pseudomonas frederiksbergensis sp. nov. is proposed. The type strain is JAJ28 T (DSM 13022 T).

OriginalsprogEngelsk
Sider (fra-til)1957-1964
Antal sider8
TidsskriftInternational Journal of Systematic and Evolutionary Microbiology
Vol/bind50
Udgave nummer6
DOI
StatusUdgivet - 2000

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  • Programområde 2: Vandressourcer

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