Immunocytochemical detection and location of the membrane-bound nitrite oxidoreductase in cells of Nitrobacter and Nitrospira

Eva Spieck, Jens Aamand, Sabine Bartosch, Eberhard Bock

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    26 Citationer (Scopus)

    Abstrakt

    The intracellular location of the membrane-bound nitrite oxidoreductase of Nitrobacter hamburgensis X14 was determined by electron microscopic immunocytochemistry. Post-embedding labeling performed on ultrathin sections revealed that the enzyme was located at the cytoplasmic membrane and at the intracytoplasmic membranes. As shown by immunonegative staining of isolated membranes the labeling of the monoclonal antibodies named Hyb 153-2 and Hyb 153-3 was located at the cytoplasmic side of the membranes. Using Hyb 153-2, which recognize the α-subunit of nitrite oxidoreductase, the labeling was found at the surface of the particulate membranes. In contrast, Hyb 153-3, recognizing the β-subunit of nitrite oxidoreductase, bound preferably at the periphery of the membrane fragments. Besides Nitrobacter, Hyb 153-3 also recognized the nitrite-oxidizing system in two strains of Nitrospira. No reaction was found with Nitrospina and Nitrococcus.

    OriginalsprogEngelsk
    Sider (fra-til)71-76
    Antal sider6
    TidsskriftFEMS Microbiology Letters
    Vol/bind139
    Udgave nummer1
    DOI
    StatusUdgivet - 15 maj 1996

    Emneord

    • Immunoassay
    • ammonium
    • oxidation
    • ammonia oxidation

    Programområde

    • Programområde 2: Vandressourcer

    Fingeraftryk

    Dyk ned i forskningsemnerne om 'Immunocytochemical detection and location of the membrane-bound nitrite oxidoreductase in cells of Nitrobacter and Nitrospira'. Sammen danner de et unikt fingeraftryk.

    Citationsformater