Fungal PAH-metabolites resist mineralization by soil microorganisms

Stine N. Schmidt, Jan H. Christensen, Anders R. Johnsen

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

36 Citationer (Scopus)

Abstrakt

This study investigated the mineralization of water-soluble polycyclic aromatic hydrocarbon (PAH) metabolites produced by the soil fungus Cunninghamella elegans. Eleven soil fungi were screened for their ability to metabolize 14C-phenanthrene, 14C-fluoranthene, and 14C-pyrene into water-soluble compounds. Eight fungi produced water-soluble metabolites from all or some of the PAHs. The composition of the water-soluble PAH-metabolites from the most effective solubilizer C. elegans was analyzed by an ultraperformance liquid chromatograph interfaced to a quadrupole time-of-flight mass spectrometer. Thirty-eight metabolites were detected. All of 34 identified metabolites were sulfate-conjugated. The mineralization of 14C-metabolites, produced by C. elegans, was compared to mineralization of the parent 14C-PAHs in soil slurries. It was hypothesized that the increased bioavailability and metabolic activation of the metabolites would increase mineralization in soil slurries compared to mineralization of the parent PAHs. Unexpectedly, the mineralization of the 14C-metabolites was in all cases extremely slow compared to the mineralization of the parent 14C-PAHs. Slow 14C-metabolite mineralization was not caused by metabolite toxicity, neither was cometabolic mineralization of 14C-metabolites stimulated by the presence of active PAH-degraders. High water solubility, low lipophilicity, and extremely slow mineralization of the metabolites indicate a potential problem of leaching of fungal PAH-metabolites to the groundwater.
OriginalsprogEngelsk
Sider (fra-til)1677-1682
Antal sider6
TidsskriftEnvironmental Science & Technology
Vol/bind44
Udgave nummer5
DOI
StatusUdgivet - 1 mar. 2010

Programområde

  • Programområde 2: Vandressourcer

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