Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen; Jim Rasmussen; Carsten S. Jacobsen; Ole S. Jacobsen; René K. Juhler; Jens Aamand

doi:10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen
, Jim Rasmussen
, Carsten S. Jacobsen
, Ole S. Jacobsen
, René K. Juhler
, Jens Aamand

Afdeling for Geokemi

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

65 Citationer (Scopus)

Resumé

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- ¹⁴C]linuron completely to ¹⁴CO₂ and ¹⁴C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Originalsprog	Engelsk
Sider (fra-til)	4144-4148
Antal sider	5
Tidsskrift	Applied and Environmental Microbiology
Vol/bind	71
Udgave nummer	7
DOI	https://doi.org/10.1128/AEM.71.7.4144-4148.2005
Status	Udgivet - jul. 2005

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Programområde 2: Vandressourcer

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10.1128/AEM.71.7.4144-4148.2005Licens: Ikke-specificeret

Citationsformater

Sorensen, S. R., Rasmussen, J., Jacobsen, C. S., Jacobsen, O. S., Juhler, R. K., & Aamand, J. (2005). Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology, 71(7), 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

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title = "Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation",

abstract = "A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.",

author = "Sorensen, \{Sebastian R.\} and Jim Rasmussen and Jacobsen, \{Carsten S.\} and Jacobsen, \{Ole S.\} and Juhler, \{Ren{\'e} K.\} and Jens Aamand",

year = "2005",

month = jul,

doi = "10.1128/AEM.71.7.4144-4148.2005",

language = "English",

volume = "71",

pages = "4144--4148",

journal = "Applied and Environmental Microbiology",

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Sorensen, SR, Rasmussen, J, Jacobsen, CS, Jacobsen, OS, Juhler, RK & Aamand, J 2005, 'Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation', Applied and Environmental Microbiology, bind 71, nr. 7, s. 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. / Sorensen, Sebastian R.; Rasmussen, Jim; Jacobsen, Carsten S. et al.
I: Applied and Environmental Microbiology, Bind 71, Nr. 7, 07.2005, s. 4144-4148.

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

TY - JOUR

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AU - Sorensen, Sebastian R.

AU - Rasmussen, Jim

AU - Jacobsen, Carsten S.

AU - Jacobsen, Ole S.

AU - Juhler, René K.

AU - Aamand, Jens

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AB - A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

UR - https://www.scopus.com/pages/publications/22144463550

U2 - 10.1128/AEM.71.7.4144-4148.2005

DO - 10.1128/AEM.71.7.4144-4148.2005

M3 - Article

SN - 0099-2240

VL - 71

SP - 4144

EP - 4148

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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Sorensen SR, Rasmussen J, Jacobsen CS, Jacobsen OS, Juhler RK, Aamand J. Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology. 2005 jul.;71(7):4144-4148. doi: 10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

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