Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen; Jim Rasmussen; Carsten S. Jacobsen; Ole S. Jacobsen; René K. Juhler; Jens Aamand

doi:10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R. Sorensen, Jim Rasmussen, Carsten S. Jacobsen, Ole S. Jacobsen, René K. Juhler, Jens Aamand

Afdeling for Geokemi

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

65 Citationer (Scopus)

Resumé

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- ¹⁴C]linuron completely to ¹⁴CO₂ and ¹⁴C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Originalsprog	Engelsk
Sider (fra-til)	4144-4148
Antal sider	5
Tidsskrift	Applied and Environmental Microbiology
Vol/bind	71
Udgave nummer	7
DOI	https://doi.org/10.1128/AEM.71.7.4144-4148.2005
Status	Udgivet - jul. 2005

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Programområde 2: Vandressourcer

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10.1128/AEM.71.7.4144-4148.2005Licens: Ikke-specificeret

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Sorensen, S. R., Rasmussen, J., Jacobsen, C. S., Jacobsen, O. S., Juhler, R. K., & Aamand, J. (2005). Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology, 71(7), 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

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title = "Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation",

abstract = "A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.",

author = "Sorensen, {Sebastian R.} and Jim Rasmussen and Jacobsen, {Carsten S.} and Jacobsen, {Ole S.} and Juhler, {Ren{\'e} K.} and Jens Aamand",

year = "2005",

month = jul,

doi = "10.1128/AEM.71.7.4144-4148.2005",

language = "English",

volume = "71",

pages = "4144--4148",

journal = "Applied and Environmental Microbiology",

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publisher = "American Society for Microbiology",

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Sorensen, SR, Rasmussen, J, Jacobsen, CS, Jacobsen, OS, Juhler, RK & Aamand, J 2005, 'Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation', Applied and Environmental Microbiology, bind 71, nr. 7, s. 4144-4148. https://doi.org/10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. / Sorensen, Sebastian R.; Rasmussen, Jim; Jacobsen, Carsten S. et al.
I: Applied and Environmental Microbiology, Bind 71, Nr. 7, 07.2005, s. 4144-4148.

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

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AU - Sorensen, Sebastian R.

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AU - Jacobsen, Ole S.

AU - Juhler, René K.

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AB - A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N′-methoxy-N′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U- 14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

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Sorensen SR, Rasmussen J, Jacobsen CS, Jacobsen OS, Juhler RK, Aamand J. Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation. Applied and Environmental Microbiology. 2005 jul.;71(7):4144-4148. doi: 10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

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