Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

Carsten S. Jacobsen

doi:10.1007/978-1-4020-2177-0_2

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

Carsten S. Jacobsen

Afdeling for Geokemi

Publikation: Bidrag til bog/rapport/konferenceproceedings › Bogkapitel › Forskning › peer review

Resumé

Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil
microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

Originalsprog	Engelsk
Titel	Molecular microbial ecology manual (Second edition)
Redaktører	George A. Kowalchuk, Frans J. de Bruijn, Ian M. Head, Antoon D.L. Akkermans, Jan Dirk van Elsas
Udgivelsessted	Dordrecht, The Netherlands
Forlag	Kluwer Academic Publishers
Kapitel	2.08
Sider	333-344
Antal sider	12
Udgave	2
ISBN (Elektronisk)	978-1-4020-2177-0
ISBN (Trykt)	978-1-4020-2176-3
DOI	https://doi.org/10.1007/978-1-4020-2177-0_2
Status	Udgivet - 2004

Programområde

Programområde 2: Vandressourcer

Adgang til dokumentet

10.1007/978-1-4020-2177-0_2

Citationsformater

Jacobsen, C. S. (2004). Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. I G. A. Kowalchuk, F. J. de Bruijn, I. M. Head, A. D. L. Akkermans, & J. D. van Elsas (red.), Molecular microbial ecology manual (Second edition) (2 udg., s. 333-344). Kluwer Academic Publishers. https://doi.org/10.1007/978-1-4020-2177-0_2

Jacobsen, Carsten S. / Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. Molecular microbial ecology manual (Second edition). red. / George A. Kowalchuk ; Frans J. de Bruijn ; Ian M. Head ; Antoon D.L. Akkermans ; Jan Dirk van Elsas. 2. udg. Dordrecht, The Netherlands : Kluwer Academic Publishers, 2004. s. 333-344

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abstract = "Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.",

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Jacobsen, CS 2004, Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. i GA Kowalchuk, FJ de Bruijn, IM Head, ADL Akkermans & JD van Elsas (red), Molecular microbial ecology manual (Second edition). 2 udg, Kluwer Academic Publishers, Dordrecht, The Netherlands, s. 333-344. https://doi.org/10.1007/978-1-4020-2177-0_2

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization. / Jacobsen, Carsten S.
Molecular microbial ecology manual (Second edition). red. / George A. Kowalchuk; Frans J. de Bruijn; Ian M. Head; Antoon D.L. Akkermans; Jan Dirk van Elsas. 2. udg. Dordrecht, The Netherlands: Kluwer Academic Publishers, 2004. s. 333-344.

Publikation: Bidrag til bog/rapport/konferenceproceedings › Bogkapitel › Forskning › peer review

TY - CHAP

T1 - Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

AU - Jacobsen, Carsten S.

PY - 2004

Y1 - 2004

N2 - Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

AB - Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.

U2 - 10.1007/978-1-4020-2177-0_2

DO - 10.1007/978-1-4020-2177-0_2

M3 - Chapter in book

SN - 978-1-4020-2176-3

SP - 333

EP - 344

BT - Molecular microbial ecology manual (Second edition)

A2 - Kowalchuk, George A.

A2 - de Bruijn, Frans J.

A2 - Head, Ian M.

A2 - Akkermans, Antoon D.L.

A2 - van Elsas, Jan Dirk

PB - Kluwer Academic Publishers

CY - Dordrecht, The Netherlands

ER -

Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

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