Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization

Carsten S. Jacobsen

Publikation: Bidrag til bog/rapport/konferenceproceedingsBogkapitelForskningpeer review

Abstrakt

Methods to extract high purity DNA directly from soil microorganisms are now easily available, allowing the application of direct hybridization techniques in soil
microbiology. Methods that can detect specific gene sequences, whether present in dead or functioning bacteria, or even as free DNA in the soil, have been developed. To achieve a sensitive detection, the choice has often been to use the polymerase chain reaction [9] to amplify the target gene in a separate step between DNA extraction and subsequent hybridization steps [12, 14]. The PCR approach is described in chapters 2.10, 2.11 and 3.1 in this manual, and a solution to solve the important problem of quantitative analysis using PCR is described in chapter 2.11, 2.13, 2.14 and 2.15.
OriginalsprogEngelsk
TitelMolecular microbial ecology manual (Second edition)
RedaktørerGeorge A. Kowalchuk, Frans J. de Bruijn, Ian M. Head, Antoon D.L. Akkermans, Jan Dirk van Elsas
UdgivelsesstedDordrecht, The Netherlands
ForlagKluwer Academic Publishers
Kapitel2.08
Sider333-344
Antal sider12
Udgave2
ISBN (Elektronisk)978-1-4020-2177-0
ISBN (Trykt)978-1-4020-2176-3
DOI
StatusUdgivet - 2004

Programområde

  • Programområde 2: Vandressourcer

Fingeraftryk

Dyk ned i forskningsemnerne om 'Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization'. Sammen danner de et unikt fingeraftryk.

Citationsformater