TY - JOUR
T1 - Cyst-theca relationships and phylogenetic positions of Peridiniales (Dinophyceae) with two anterior intercalary plates, with description of Archaeperidinium bailongense sp nov and Protoperidinium fuzhouense sp nov.
AU - Liu, Tingting
AU - Mertens, Kenneth Neil
AU - Ribeiro, Sofia
AU - Ellegaard, Marianne
AU - Matsuoka, Kazumi
AU - Gu, Haifeng
N1 - Publisher Copyright:
© 2015 Japanese Society of Phycology.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Protoperidinium species with two anterior intercalary plates were originally classified by Jörgensen in the subgenus Archaeperidinium and assigned to the sections Excentrica, Avellana and Archaeperidinium
by Taylor, on the basis of the relative size of anterior intercalary
plates and the extent of cingulum displacement. Phylogenetic
relationships among these three sections have not been fully explored.
Recently, Archaeperidinium was reinstated as a genus, but several
species fitting the emended description have not been transferred
formally as molecular data were not available. In the present study we
examined the cyst-theca relationship of seven species with two anterior
intercalary plates: four species assigned to Protoperidinium and three to Archaeperidinium,
from the China Sea. Partial large subunit ribosomal DNA sequences were
obtained from these seven species by single-cell polymerase chain
reaction, and for the cyst of Protoperidinium stellatum from France. Two new species, Archaeperidinium bailongense and Protoperidinium fuzhouense, were described based on both theca and cyst morphology, and the cyst-theca relationships of A. constrictum and P. abei var. rotunda were established for the first time. Maximum-likelihood and Bayesian inference analyses revealed that P. fuzhouense was nested within the Protoperidinium sensu stricto clade despite having only six postcingular plates, and sections Excentrica, Avellana and the genus Archaeperidinium
were all monophyletic. Our results suggest that the presence/absence of
a sulcal fin and antapical horns and the displacement of the cingulum
are the most stable characteristics of the motile stages within the
respective clades.
AB - Protoperidinium species with two anterior intercalary plates were originally classified by Jörgensen in the subgenus Archaeperidinium and assigned to the sections Excentrica, Avellana and Archaeperidinium
by Taylor, on the basis of the relative size of anterior intercalary
plates and the extent of cingulum displacement. Phylogenetic
relationships among these three sections have not been fully explored.
Recently, Archaeperidinium was reinstated as a genus, but several
species fitting the emended description have not been transferred
formally as molecular data were not available. In the present study we
examined the cyst-theca relationship of seven species with two anterior
intercalary plates: four species assigned to Protoperidinium and three to Archaeperidinium,
from the China Sea. Partial large subunit ribosomal DNA sequences were
obtained from these seven species by single-cell polymerase chain
reaction, and for the cyst of Protoperidinium stellatum from France. Two new species, Archaeperidinium bailongense and Protoperidinium fuzhouense, were described based on both theca and cyst morphology, and the cyst-theca relationships of A. constrictum and P. abei var. rotunda were established for the first time. Maximum-likelihood and Bayesian inference analyses revealed that P. fuzhouense was nested within the Protoperidinium sensu stricto clade despite having only six postcingular plates, and sections Excentrica, Avellana and the genus Archaeperidinium
were all monophyletic. Our results suggest that the presence/absence of
a sulcal fin and antapical horns and the displacement of the cingulum
are the most stable characteristics of the motile stages within the
respective clades.
KW - Archaeperidinium constrictum
KW - Archaeperidinium minutum
KW - Cyst-theca relationship
KW - Dinoflagellate cysts
KW - LSU rDNA
KW - Protoperidinium abei
KW - Protoperidinium avellana
KW - Protoperidinium excentricum
KW - Protoperidinium stellatum
UR - http://www.scopus.com/inward/record.url?scp=84928215308&partnerID=8YFLogxK
U2 - 10.1111/pre.12081
DO - 10.1111/pre.12081
M3 - Article
SN - 1322-0829
VL - 63
SP - 134
EP - 151
JO - Phycological Research
JF - Phycological Research
IS - 2
ER -