Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

J. F. Schwensen, C. Menné Bonefeld, C. Zachariae, C. Agerbeck, T. H. Petersen, C. Geisler, U. E. Bollmann, K. Bester, J. D. Johansen

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

24 Citationer (Scopus)

Abstrakt

Background: In the light of the exceptionally high rates of contact allergy to the preservative methylisothiazolinone (MI), information about cross-reactivity between MI, octylisothiazolinone (OIT) and benzisothiazolinone (BIT) is needed. Objectives: To study cross-reactivity between MI and OIT, and between MI and BIT. Methods: Immune responses to MI, OIT and BIT were studied in vehicle and MI-sensitized female CBA mice by a modified local lymph node assay. The inflammatory response was measured by ear thickness, cell proliferation of CD4+ and CD8+ T cells, and CD19+ B cells in the auricular draining lymph nodes. Results: MI induced significant, strong, concentration-dependent immune responses in the draining lymph nodes following a sensitization phase of three consecutive days. Groups of MI-sensitized mice were challenged on day 23 with 0·4% MI, 0·7% OIT and 1·9% BIT – concentrations corresponding to their individual EC3 values. No statistically significant difference in proliferation of CD4+ and CD8+ T cells was observed between mice challenged with MI compared with mice challenged with BIT and OIT. Conclusions: The data indicate cross-reactivity between MI, OIT and BIT, when the potency of the chemical was taken into account in choice of challenge concentration. This means that MI-sensitized individuals may react to OIT and BIT if exposed to sufficient concentrations.

OriginalsprogEngelsk
Sider (fra-til)176-183
Antal sider8
TidsskriftBritish Journal of Dermatology
Vol/bind176
Udgave nummer1
DOI
StatusUdgivet - 1 jan. 2017
Udgivet eksterntJa

Programområde

  • Programområde 5: Natur og klima
  • Programområde 2: Vandressourcer

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