Characterization of monoclonal antibodies raised against different structures belonging to the s-triazine group of herbicides

Leif Bruun, Claus Koch, Mogens Havsteen Jakobsen, Brian Pedersen, Michael Christiansen, Jens Aamand

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

26 Citationer (Scopus)

Abstrakt

Monoclonal antibodies were raised against seven haptens, representing different structures of the s-triazine group of herbicides. Two side groups on the s-triazine ring were used to couple reactive s-triazine-haptens to immunogenic carrier molecules. For each hapten, one monoclonal antibody was selected for further analysis based on its affinity for the homologous s-triazine. The selected monoclonal antibodies were characterized with respect to their reactivity to the seven immunogens in a direct ELISA assay and cross-reactivity to nine free s-triazines (atrazine, hydroxyatrazine, simazine, hydroxysimazine, terbutylazine, deisopropylatrazine, deethylatrazine, deethylterbutylazine and cyanazine) in competitive assays. The pattern of reactivities illustrates the varying antigenic dominance of the different side groups of the s-triazine ring as well as the potential of monoclonal antibodies for use in screening drinking water, etc. for the presence of s-triazine herbicides.

OriginalsprogEngelsk
Sider (fra-til)87-101
Antal sider15
TidsskriftAnalytica Chimica Acta
Vol/bind436
Udgave nummer1
DOI
StatusUdgivet - 1 jun. 2001

Emneord

  • Pesticide
  • Analytical technique
  • Immunoassay

Programområde

  • Programområde 2: Vandressourcer

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