TY - JOUR
T1 - Biostimulation and enrichment of 2,6-dichlorobenzamide-mineralising soil bacterial communities from dichlobenil-exposed soil
AU - Holtze, Maria S.
AU - Sørensen, Sebastian R.
AU - Sørensen, Jan
AU - Hansen, Hans Christian B.
AU - Aamand, Jens
N1 - Funding Information:
This study was supported by the Danish Technical Research Council (the Immunalyse Project and funding for S.R. Sørensen) and the Danish Research Council (the RECETO research school and the Pesticon project). We thank Mette Andersen for skilful technical assistance with the DGGE fingerprinting.
PY - 2007/1
Y1 - 2007/1
N2 - Eleven enrichment cultures of 2,6-dichlorobenzamide (BAM)-mineralising bacteria from a dichlobenil-exposed soil were enriched using three different media. Ten of the enriched mixed cultures had stimulated BAM mineralisation only in the presence of supplementary carbon and were able to utilise BAM as the sole nitrogen source, while only one was able to utilise BAM as the sole source of both carbon and nitrogen. Cultivation and DNA-based analysis of the mixed cultures suggested that different bacterial populations were stimulated by the three strategies. Our findings indicate that natural BAM-mineralising populations capable of using BAM as a source of nitrogen can be readily stimulated, enriched and maintained following sub-culturing from dichlobenil-treated soils by adding an alternative carbon source.
AB - Eleven enrichment cultures of 2,6-dichlorobenzamide (BAM)-mineralising bacteria from a dichlobenil-exposed soil were enriched using three different media. Ten of the enriched mixed cultures had stimulated BAM mineralisation only in the presence of supplementary carbon and were able to utilise BAM as the sole nitrogen source, while only one was able to utilise BAM as the sole source of both carbon and nitrogen. Cultivation and DNA-based analysis of the mixed cultures suggested that different bacterial populations were stimulated by the three strategies. Our findings indicate that natural BAM-mineralising populations capable of using BAM as a source of nitrogen can be readily stimulated, enriched and maintained following sub-culturing from dichlobenil-treated soils by adding an alternative carbon source.
KW - 2,6-dichlorobenzamide
KW - BAM
KW - Biodegradation
KW - Biostimulation
KW - Dichlobenil
UR - http://www.scopus.com/inward/record.url?scp=33750953323&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2006.07.009
DO - 10.1016/j.soilbio.2006.07.009
M3 - Article
VL - 39
SP - 216
EP - 223
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
IS - 1
ER -