A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format

Anders R. Johnsen; Trine K. Hybholt; Ole S. Jacobsen; Jens Aamand

doi:10.1016/j.mimet.2009.07.018

A radiorespirometric method for measuring mineralization of [¹⁴C]-compounds in a 96-well microplate format

Anders R. Johnsen, Trine K. Hybholt, Ole S. Jacobsen, Jens Aamand

Afdeling for Geokemi

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

17 Citationer (Scopus)

Resumé

A radiorespirometric method was developed for quantifying mineralization of [¹⁴C]-labeled substrates in deep-well microplates. Ninety-six ¹⁴CO₂-traps were placed on an adhesive microplate sealing tape which made it possible to trap ¹⁴CO₂ simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

Originalsprog	Engelsk
Sider (fra-til)	114-116
Antal sider	3
Tidsskrift	Journal of Microbiological Methods
Vol/bind	79
Udgave nummer	1
DOI	https://doi.org/10.1016/j.mimet.2009.07.018
Status	Udgivet - okt. 2009

Programområde

Programområde 2: Vandressourcer

Adgang til dokumentet

10.1016/j.mimet.2009.07.018

Citationsformater

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title = "A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format",

abstract = "A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.",

keywords = "Autoradiography, Mineralization, MPN, Phenanthrene, Soil",

author = "Johnsen, \{Anders R.\} and Hybholt, \{Trine K.\} and Jacobsen, \{Ole S.\} and Jens Aamand",

note = "Funding Information: This work was funded by the Villum Kann Rasmussen Foundation via the Center for Environmental and Agricultural Microbiology (CREAM).",

year = "2009",

month = oct,

doi = "10.1016/j.mimet.2009.07.018",

language = "English",

volume = "79",

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journal = "Journal of Microbiological Methods",

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T1 - A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format

AU - Johnsen, Anders R.

AU - Hybholt, Trine K.

AU - Jacobsen, Ole S.

AU - Aamand, Jens

N1 - Funding Information: This work was funded by the Villum Kann Rasmussen Foundation via the Center for Environmental and Agricultural Microbiology (CREAM).

PY - 2009/10

Y1 - 2009/10

N2 - A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

AB - A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

KW - Autoradiography

KW - Mineralization

KW - MPN

KW - Phenanthrene

KW - Soil

UR - http://www.scopus.com/inward/record.url?scp=70349216333&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2009.07.018

DO - 10.1016/j.mimet.2009.07.018

M3 - Article

C2 - 19635506

SN - 0167-7012

VL - 79

SP - 114

EP - 116

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1