TY - JOUR
T1 - A quantitative enzyme-linked immunoassay for the detection of 2,6- dichlorobenzamide (BAM), a degradation product of the herbicide dichlobenil
AU - Bruun, Leif
AU - Koch, Claus
AU - Pedersen, Brian
AU - Jakobsen, Mogens Havsteen
AU - Aamand, Jens
N1 - Funding Information:
Eva Stenbæk Jauho, Mikkel Nørholm and Ulrik Boas are thanked for valuable suggestions and discussions. Spire Maja Kiersgaard and Karina Thaulov are thanked for skilled technical assistance. The project was financed by the Ministry of Environment and Energy.
PY - 2000/6/23
Y1 - 2000/6/23
N2 - 2,6-Dichlorobenzamide (BAM) is the dominant degradation product in soil of the widely used herbicide dichlobenil. To detect BAM in water, a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) was developed. As an alternative to conventional coating of ELISA plates, the assay is based on direct covalent immobilisation. We achieved a surface which requires a short time for the immobilisation of ligand, is stable under dry storage, and which permits assays with a low CV. The performance of the assay was demonstrated by an inter-well CV that was generally less than 6%, a detection limit (DL15) of 0.02 μg/l and an IC50 of 0.19 μg/l. Cross- reactivity was measured against nine analytes with structural homology to BAM. The highest degree of cross-reactivity (10.8%) was seen with 2,6- dichlorothiobenzamide (Chlorthiamid). Considering an EU-limit of 0.1 μg/l as the permissible maximum for the presence of pesticides in drinking water, this ELISA-procedure is suitable for large-scale screening of water samples suspected of being contaminated with BAM. (C) 2000 Elsevier Science B.V.
AB - 2,6-Dichlorobenzamide (BAM) is the dominant degradation product in soil of the widely used herbicide dichlobenil. To detect BAM in water, a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) was developed. As an alternative to conventional coating of ELISA plates, the assay is based on direct covalent immobilisation. We achieved a surface which requires a short time for the immobilisation of ligand, is stable under dry storage, and which permits assays with a low CV. The performance of the assay was demonstrated by an inter-well CV that was generally less than 6%, a detection limit (DL15) of 0.02 μg/l and an IC50 of 0.19 μg/l. Cross- reactivity was measured against nine analytes with structural homology to BAM. The highest degree of cross-reactivity (10.8%) was seen with 2,6- dichlorothiobenzamide (Chlorthiamid). Considering an EU-limit of 0.1 μg/l as the permissible maximum for the presence of pesticides in drinking water, this ELISA-procedure is suitable for large-scale screening of water samples suspected of being contaminated with BAM. (C) 2000 Elsevier Science B.V.
KW - 2,6-Dichlorobenzamide
KW - Antibody
KW - BAM
KW - Covalent immobilisation
KW - Dichlobenil
KW - Herbicide
KW - Immunoassay
KW - Pesticide
KW - Pesticide
KW - Immunoassay
UR - http://www.scopus.com/inward/record.url?scp=0034705719&partnerID=8YFLogxK
U2 - 10.1016/S0022-1759(00)00190-3
DO - 10.1016/S0022-1759(00)00190-3
M3 - Article
C2 - 10854608
AN - SCOPUS:0034705719
VL - 240
SP - 133
EP - 142
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -