A new monoclonal antibody for the sensitive detection of cyanazine and other s-triazines in water by ELISA

Leif Bruun, Claus Koch, Mogens Havsteen Jakobsen, Jens Aamand

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

10 Citationer (Scopus)

Abstrakt

A new monoclonal antibody with specificity for s-triazine herbicides was prepared using a tertbutyl-s-triazine hapten. An assay was developed, employing covalently immobilized hapten and monoclonal antibody labeled with horseradish peroxidase. This assay system had a detection limit of 0.01 μgl-1 cyanazine, with a coefficient of variation less than 3%. Analysis on three different spiked water types showed an average recovery of 101% at 0.1 μgl-1 cyanazine. Cross-reactions were found for atrazine, terbuthylazine and propazine in the range 18-62%. This immunoassay is therefore capable of detecting four different s-triazine herbicides at concentrations well below the limit value for drinking water of 0.1 μgl-1 stipulated by the EEC Council Directive on drinking water.

OriginalsprogEngelsk
Sider (fra-til)253-262
Antal sider10
TidsskriftFood and Agricultural Immunology
Vol/bind12
Udgave nummer4
DOI
StatusUdgivet - 2000

Emneord

  • Immunoassay
  • Pesticide

Programområde

  • Programområde 2: Vandressourcer

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